Methylene Blue 4% Solution

What is Methylene Blue 4% Solution? It is an aqueous solution of USP(Pharmaceutical) grade methylene blue, a synthetic dye with the chemical formula C16H18ClN3S, dissolved at a concentration of 4% in high purity distilled water.

It is widely used in laboratories and at home for various applications, including staining cells, tissues, and microorganisms to enhance their visibility under a microscope. Methylene Blue is also employed as a redox indicator in titrations, where it changes colour based on the oxidation state of the environment. 

The Original Additive Free Methylene Blue Solution: We were the first to see the need for an additive free commercially available Methylene Blue solution. Our Methylene Blue is purely pharmaceutical grade Methylene Blue powder and grade 1 purity distilled water. No additives or preservatives and full clarity for our customers.

High-Quality Formulation: Crafted with carefully sourced USP grade Methylene Blue powder and grade 1 distilled water, our solution is made with high-quality ingredients to ensure safe and effective use.

For more information read our blog on What is USP Grade? A Methylene Blue 1% Case Study

Manufactured in the UK: All of our Methylene Blue Solutions are manufactured and bottled in the UK. Using non-toxic, chemical-resistant bottles, providing reliable and trusted quality to our customers.

Dispensing and Bulk Options Available: A 100ml pipette option is available for convenient dispensing, and pack sizes go from 50ml all the way up to 1L 

UV Resistant Amber Glass Bottles

All our Methylene Blue solutions come in UV resistant amber glass bottles. These are much more UV resistant then the blue glass alternatives.

Storage

Store in a cool dry place out of direct sunlight

Frequently Asked Questions:

For more information read our Blog The Chemical Properties of Methylene Blue

How much should I take?

Although we specialise in producing specialist solutions, we are not medically trained. If looking to ingest any product we sell we would recommend seeking the advice of a medical professional before purchasing. We are supply only so usage comes down to yourselves to use responsibly.

Why do the photos look like the liquid is brown/clear?

The bottles are UV resistant amber glass, they'll look brown even when filled. Our images are taken with water so you can see the internal bottle structure/pipette. The sent product will contain the actual solution which is a dark blue in colour.

Should I get the solution or the powder?

This is completely your decision and purely depends upon how much you like doing yourself. Methylene Blue is messy so the solution can offer a cleaner option at a controlled volume/percentage. If you like to have more personal control over solutions the powder is a great option with the correct settup and storage.

How long will my solution last?

If kept cool and out of sunlight an unopened solution will last a long time. Once opened its worth being aware microbes can grow in the solution if contaminated. Avoid introducing contaminants and follow storage instructions to give the best life to your solution. If you notice things growing in the solution we would recommend following appropriate disposal of any waste and purchasing a fresh batch.

How do I clean up spilled Methylene Blue?

Methylene Blue is a Dye, it's primary function is to colour organic matter. As such it's messy if spilled! The best thing we've found to clean it (depending on surface, always test first) is Alcohol most commonly IPA(isopropyl alcohol). 

How do I stop the dyeing effect?

While we can't give specifics Vitamin C has been shown to neutralise the strong blue colour of Methylene Blue.

How Transparent are we?

We're a long established UK based manufacturer and retailer of stains, solutions and much more. Transparency is core to our business, we don't like nasty surprises and we don't think you should have them either. Our Methylene Blue solution is produced in house, with our certified Methylene blue powder coming direct from manufacturer and our grade 1 distilled water manufactured right here on site.

Is our Methylene Blue Organic/Non GMO?

Methylene Blue is an artifical dry dye, it is manufactured so if you see this on products its likely about as much use as us saying its vegan 

I have sediment at the bottom of my solution?

Methylene Blue in a 4% solution is close to the max soluble amount in water, as such you may find after a period of rest some dye comes out of solution. If this is the case give it a good shake whilst firmly closed and this should mix it thoroughly for use. If the solution has been open for a while or stored incorrectly and the particles are floating before shaking it may have spoiled and should be replaced where possible.

• laboratories staining
• redox indicator in titrations

Contains:
USP Grade Methylene Blue: <4%
Distilled Water

Please note, the below is in a 4% solution. 1ppm would roughly equate to 0.04ppm in a 4% solution

Each drop contains roughly 2.0 mg USP grade methylene blue powder.

Powder Specification

Conforms to USP Specification

Assay (Dry Basis): 98.0-103.0%
Molecular Formula: C₁₆H₁₈CIN₃S*3H₂O
Molecular Weight: 373.90
Loss on drying: 13.74%
Residue on ignition: <0.84%wt
Arsenic (As): <2.15ppm
Copper (Cu): <55ppm
Zinc (Zn): No Turbidity within 5 min

Residual Solvents:

Methylene Chloride: <126 ug/g
Chloroform: <12 ug/g
Trichloroethylene: <15 ug/g
1,4-Dioxane: <49 ug/g

HS Code / Commodity Code: 3822900000

Hazard Phrases: H0001